HPLC-Charged Aerosol Detection Surfactants and Emulsifiers



Surfactants are a diverse group of chemicals whose structures vary widely
but typically contain an oil-soluble hydrocarbon chain and a water-soluble
hydrophilic group. Surfactants can be categorized based upon their
structure and include nonionic, anionic, and cationic classes. They have
widespread use as detergents in shampoos and cleaning products, ion
pairing agents used in chromatography, and complex dispersants used
to treat oil spills. Emulsifiers are used to maintain a uniform suspension of
immiscible materials. These compounds are typically surfactants, and can
be designed for use in specific applications and products in both the food
and pharmaceutical industries. Many of these commercial surfactants
are mixtures of members of a homologous series (often referred to as
congeners), and such mixtures can be defined or characterized using LC.
Chromatographic approaches can be used to separate the molecules on
the basis of carbon chain length, chain branching, or positional isomer
distribution. This is important, for example, when studying lot-to-lot
variability, which can be important to the biopharma industry. Conversely,
when trying to quantitate total amounts of surfactants or emulsifiers, the
chromatographic conditions need to be changed so that all the congeners
elute as a single peak, thus simplifying the determination.


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Using our UHPLC-ready systems, highly sensitive and selective detectors,
and state of the art column technologies, along with proven analytical
methods, precise automation and advanced data handling will help you to:
• Characterize or quantify many typical classes of surfactants
• Analyze compounds in a broad range of samples
• Implement simplified methods with improved sensitivity
and reproducibility

For surfactant analysis using high-sensitivity detection, the column of
choice is the Thermo Scientific™ Acclaim™ Surfactant Plus LC column.
This new generation of high-efficiency silica-based columns features
exceptionally low bleed for use with charged aerosol detectors and mass
spectrometers. The columns provide excellent selectivity for sensitive
separations of a wide variety of surfactants. The advanced surface
chemistry provides both reversed-phase and anion-exchange retention
mechanisms and significantly improves the resolution of surfactants.

The Acclaim Surfactant Plus LC column provides improved performance,
versatility and throughput for surfactant analysis
• Ideal selectivity for simultaneous separation of anionic, nonionic,
cationic, and amphoteric surfactants
• Resistant to dewetting under highly aqueous mobile phase conditions
• Excellent resolution between strongly hydrophilic compounds
• Rugged separation under a variety of conditions

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Continue at: https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/AI-71104-HPLC-CAD-Surfactants-Emulsifiers-Applications-Notebook-AN71104-EN.pdf

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